virulence. Larval mortality was dose dependent for all EPN species. When using a dose of 100 infective juveniles (IJs) per larva, H. bacteriophora and S. carpocapsae caused 90%-100% mortality, whereas H. downesi and S. feltiae caused only 40%-60% mortality. Even when using 200 IJs/larva, H. megidis and S. kraussei caused a maximum of 30%-40% mortality. Some of the invasive EPNs were melanized, suggesting a strong humoral defense response by the Aedes larvae. The degree of melanization was quite variable; some EPNs were totally enveloped in a melanin sheath while others were partially coated with melanin. Melanization did not stop the EPN from multiplying and killing the Aedes larvae. IJs released from infected larvae would have the potential to infect healthy mosquito larvae. Also, both bacterial supernatant and bacterial cell suspension of Xenorhabdus nematophila caused >91% larval mortality after 48 h, whereas only the bacterial cell suspension of Photorhabdus laumondii was effective against the mosquito larvae. These data provides useful information on the potential use of EPNs and/or formulated bacterial cell suspensions in the control of the important urban and container-breeding mosquito, Ae. aegypti, and are a starting point for future simulated and actual field studies.

Keywords

Entomopathogenic nematode (EPN) ingestion, Aedes aegypti, symbiotic bacteria, H. bacteriophora, S. carpocapsae

2017 and 2018 at Beni-Suef governorate. Samples from diseased cattle, previously SPPV vaccinated with SPPV, were inoculated in embryonated chicken eggs (ECE) using chorio-allantoic membrane (CAM) route. Histopathological examination of the inoculated CAMs showed large eosinophilic intracytoplasmic inclusion bodies characteristic for LSDV. Characteristic pock lesions were observed after the 1st passage and become clear after the third passage and become clearly observed 4 days post inoculation. For molecular identification, DNA was extracted from a pool of the infected CAMs. Two pairs of primers specific for LSDV including one flanking a 554 bp product of the G gene and second flanking 172 of the RP030 gene were used. In conclusion, LSDV infections have been detected and the virus has been isolated and identified by PCR from cattle previously vaccinated with SPPV vaccine during the summer of 2018

Prosopis farcta (Banks & Sol.) J. F. Macbr. has been reported for the first time from Libya. This species is native in Asia. Flowering specimens of Prosopis farcta collected from Sokna Region about 650 Km south-east Tripoli. It is presented with updated nomenclature, taxonomic description, geographical distribution, place of occurrence in Libya, and field colored photographs are also provided. The voucher specimens were deposited in the Herbarium of Botany Department (ULT), University of , Tripoli, Libya.

Shhoob Mohamed imhmed Elahmir, (07-2021), UNITED STATES: تم اختياره Quest Journals, 8 (6), 39-43

A floristic survey of Sedrores Al-Qawasim mountains in Gharyan district - Libya was conducted in two consecutive growing seasons from 01/04/2018 to 01/6/2019. In this study area, a total number of 320 plant species belonging to 214 genera were collected representing 44 families, of which 39 families and 276 species belonging to dicotyledons, and 5 families and 44 species are belonging to monocotyledons. The results showed the dominance of the family Asteraceae with 60 species, followed by the family Fabaceae with 32 species, then the family Poaceae with 29 species. The results showed the dominance of the (Euphorbia genus) with 7 species, followed by Helianthenum, Plantago and Erodium with 6 species each. Moreover, life-form spectrum analysis showed the predominance of therophytes with 210, followed by Hemicryptophytes with 59 species, while chorotype spectrum analysis showed the dominance of Mediterranian species with 151 species, followed by Mediterrean/Iranu-Turanean with 72 species. 

Shhoob Mohamed imhmed Elahmir, Mohammed H. Mahklouf, Mahmood B. Shanta, Hisham Ali Abo -Jaafer, (07-2020), كندا: J. of Advanced Botany and Zoology, 1 (8), 1-10

A new record for Abutilon theophrasti is recorded for the first time in the flora of Libya. This widespread agricultural weed was collected from El-Hamra region (El-Qabel) in the Nafusa Mountain south of Tripoli. To facilitate further identification and future detection, a full description, habitat information, distribution map and distribution data are provided. A brief discussion about the most important threats posed by this species is presented.

Shhoob Mohamed imhmed Elahmir, MOHAMMED. H. MAHKLOUF, (05-2019), ابريطانيا: Phytotaxa, 402 (5), 259-254

Currently the origin and trajectories of novel traits are emphasised in evolutionary studies, the

role of stabilization is neglected, and interpretations are often post hoc rather than as

hypothesised responses to stated agents of selection. Here we evaluated the impact of

changing environmental conditions on trait evolution and stabilization and their relative

contribution to diversification in a prominent Australian genus, Hakea (Proteaceae). We

assembled a time-based phylogeny for Hakea, reconstructed its ancestral traits for six

attributes and determined their evolutionary trajectories in response to the advent or

increasing presence of fire, seasonality, aridity, nectar-feeding birds and (in)vertebrate

herbivores/granivores. The ancestral Hakea arose 18 million years ago (Ma) and was broad

leaved, non-spinescent, insect-pollinated, had medium-sized, serotinous fruits and resprouted

after fire. Of the 190 diversification events that yielded the 82 extant species analysed, 8−50%

involved evolution, stabilization or re-evolution (reversal) of individual novel traits.

شهوب محممد أمحمد الأحمر, Byron B. Lamont, (10-2017), استراليا: تم اختياره bioRxiv Journal, 1 (10), 1-33

Six Hakea species varying greatly in seed size were selected for cotyledon damage experiments. The

growth of seedlings with cotyledons partially or completely removed was monitored over 90 days. All

seedlings perished by the fifth week when both cotyledons were removed irrespective of seed size.

Partial removal of cotyledons caused a significant delay in the emergence of the first leaf, and reduction

in root and shoot growth of the large-seeded species. The growth of seedlings of small-seeded species

was less impacted by cotyledon damage. The rate of survival, root and shoot lengths and dry biomass

of the seedlings were determined after 90 days. When seedlings were treated with balanced nutrient

solutions following removal of the cotyledons, survival was 95–98%, but 0% when supplied with

nutrient solutions lacking N or P or with water only. The addition of a balanced nutrient solution failed

to restore complete growth of any species, but the rate of root elongation for the small-seeded species

was maintained. Cotyledons provide nutrients to support early growth of Hakea seedlings, but other

physiological roles for the cotyledons are also implicated. In conclusion, small-seeded Hakea species can

tolerate cotyledons loss better than large-seeded species.